|
KMID : 0545120170270112070
|
|
Journal of Microbiology and Biotechnology
2017 Volume.27 No. 11 p.2070 ~ p.2073
|
|
|
A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase
|
|
Lee Jin-Moo
Cho Jin-Beom
Ahn Hee-Chul
Jung Woong
Jeong Yong-Joo
|
|
|
Abstract
|
|
|
|
We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC50 values of 2.09 ± 0.30 μM (ATP hydrolysis) and 13.2 ± 0.9 μM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 μM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.
|
|
|
KEYWORD
|
|
|
SARS, helicase, ATP hydrolysis, dsDNA unwinding, cytotoxicity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|